Mark Gillespie

Research Scientist

Ranish Lab

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(206) 732-1425


The interplay between lipid metabolism and inflammation is central to the pathology of numerous chronic diseases, including cardiovascular disease, diabetes, and obesity. These diseases represent an increasing global health burden. My research focuses on understanding how the intersection of these pathways influences gene expression and cellular responses in specialized cells of the immune system, known as macrophages. I use innovative quantitative mass spectrometry approaches to measure dynamic changes in transcription factor abundance and transcription factor complex assembly, and uncover novel regulatory mechanisms in macrophages. I apply this information to gain critical insights into the development and progression of cardiovascular disease due to atherosclerosis.

Gillespie MA, Gold ES, Ramsey SA, Podolsky, I, Aderem A, and Ranish JA. (2015) An LXR-NCOA5 gene regulatory complex directs inflammatory crosstalk-dependent repression of macrophage cholesterol efflux. The EMBO Journal. Epub 2015 Mar 9. pii: e201489819. PMID: 25755249

Scimé A, Soleimani VD, Bentzinger CF, Gillespie, MA, Le Grand F, Grenier G, Bevilacqua L, Harper ME, and Rudnicki MA. (2010) Oxidative status of muscle is determined by p107 regulation of PGC-1a. Journal of Cell Biology. 190:651-662. PMID: 20713602, PMCID: PMC2928004.

Gillespie MA, Le Grand F, Scime A, Kuang S, von Maltzahn J, Seale V, Cuenda A, Ranish JA, and Rudnicki MA. (2009) p38-{gamma}-dependent gene silencing restricts entry into the myogenic differentiation program. Journal of Cell Biology. 187:991-1005. PMID: 20026657, PMCID: PMC2806273.

Kuang S, Gillespie MA, and Rudnicki MA. (2008) Niche regulation of muscle satellite cell self-renewal and differentiation. Cell Stem Cell. 2:22-31. PMID: 18371418.

Scimé A, Grenier G, Huh MS, Gillespie MA, Bevilacqua L, Harper ME, and Rudnicki MA. (2005) Rb and p107 regulate preadipocyte differentiation into white versus brown fat through repression of PGC-1alpha. Cell Metabolism. 2:283-295. PMID: 16271529.

Gillespie MA, and Rudnicki MA. (2004) Something to SNF about. Nature Genetics. 36:676-677. PMID: 15226749.

Quantitative mass spectrometry, systems immunology, transcriptional regulation

PhD, Cellular and Molecular Medicine, University of Ottawa