Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae

TitleIdentification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae
Publication TypeJournal Article
Year of Publication2001
AuthorsMayer ML, Gygi SP, Aebersold R, Hieter P
JournalMol Cell
Volume7
Pagination959-70
Date PublishedMay
PMID11389843
Keywords*Homeodomain Proteins, *Proto-Oncogene Proteins c-bcl-2, *Repressor Proteins, *Saccharomyces cerevisiae Proteins, Benomyl/pharmacology, Cell Cycle/drug effects, Chromatids/metabolism/*physiology/ultrastructure, Chromosomal Proteins, Non-Histone/genetics/metabolism/pharmacology, DNA Replication, DNA-Binding Proteins/analysis/*genetics/metabolism, Fungal Proteins/genetics/metabolism, Gene Deletion, Models, Molecular, Mutation, Precipitin Tests, Protein Binding, Protein Subunits, Replication Protein C, Saccharomyces cerevisiae/*genetics
AbstractWe have identified and characterized an alternative RFC complex RFC(Ctf18p, Ctf8p, Dcc1p) that is required for sister chromatid cohesion and faithful chromosome transmission. Ctf18p, Ctf8p, and Dcc1p interact physically in a complex with Rfc2p, Rfc3p, Rfc4p, and Rfc5p but not with Rfc1p or Rad24p. Deletion of CTF18, CTF8, or DCC1 singly or in combination (ctf18Deltactf8Deltadcc1Delta) leads to sensitivity to microtubule depolymerizing drugs and a severe sister chromatid cohesion defect. Furthermore, temperature-sensitive mutations in RFC4 result in precocious sister chromatid separation. Our results highlight a novel function of the RFC proteins and support a model in which sister chromatid cohesion is established at the replication fork via a polymerase switching mechanism and a replication-coupled remodeling of chromatin.

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