ATAQS (Automated and Targeted Analysis with Quantitative SRM)

As a complement to the well-established discovery proteomic methods, targeted mass spectrometry based on SRM is becoming an important tool for the generation of reproducible, sensitive and quantitatively accurate data from biological samples. The method depends on the generation of target protein sets based on prior information and the one-time generation of verified mass spectrometric assays for each of the targeted proteins.  The development of these assays depends on the optimal selection of peptides that represent the proteins on the target list and the optimal set of transitions for their detection in biological samples. Once developed, these assays can be continually applied across a multitude of studies.